REALQUALITY RQ-EBV
Description
REALQUALITY RQ-EBV is a diagnostic CE-IVD kit for the identification and quantification of the DNA of Epstein-Barr virus (EBV) by Real time PCR.
Peculiarity
- The device is validated on DNA extracted from various samples types
- The assay requires only 5 µL of DNA extracted
- If used in combination with the REALQUALITY RQ-EBV STANDARD, it allows the quantification of the viral DNA present in the sample
- The assay shares the same thermal profile of the REALQUALITY Infectious diseases kits
- Validated on main Real time PCR instruments
- The assay includes dUTP/UNG system for preventing carry-over contamination and a fluorescence normalizer
- The automatic format of the assay can be used on GENEQUALITY X120 platform
- Easy interpretation of results with AB Genius Report software
Kit content
- Ready-to-use reagents for Real time PCR
- Internal control (amplification of beta-globin gene - BG)
- Positive control (DNA containing fragments of the EBV genome and the BG gene)
For further information
The Epstein-Barr virus (EBV), or Human Herpesvirus 4, is one of the most common viral agents in the world. It spreads mainly through saliva, causing infectious mononucleosis, which is common in the paediatric population and adolescents. Occurrence of the virus in the body stimulates abnormal production of mononuclear cells, which are normally present in the blood in small quantities.
Once contracted, the virus remains in the body for life, the B lymphocytes being the main site of latency.
The Epstein-Barr virus is believed to be a cofactor in the development of some tumours, such as Burkitt’s lymphoma, nasopharyngeal carcinoma and Hodgkin's lymphoma.
Why use Real time PCR assay for EBV?
In individuals who have undergone a solid organ transplant, EBV infection can trigger uncontrolled proliferation of B cells, known as post-transplant lymphoproliferative disease (PTLD), which poses a serious threat to the patients. However, PTLD is reversible if detected early; this is why monitoring of the viral load using the quantitative Real time (PCR) test is essential.
This product uses technology patented by Biosearch Technologies, licensed for use in Human Molecular Diagnostic applications.
