REALQUALITY RQ-MBT Complex is a diagnostic CE-IVD kit for the identification and quantification of the DNA of Mycobacterium tubercolosis complex (MBTC) by Real time PCR


  • The device is validated on DNA extracted from various samples types
  • The assay requires only 5 µL of DNA extracted
  • If used in combination with the REALQUALITY RQ-MBT Complex STANDARD, it allows the quantification of the viral DNA present in the sample 
  • The assay shares the same thermal profile of the REALQUALITY Infectious diseases kits
  • Validated on main Real time PCR instruments
  • The assay includes dUTP/UNG system for preventing carry-over contamination and a fluorescence normalizer
  • The automatic format of the assay can be used on GENEQUALITY X120 platform
  • Easy interpretation of results with AB Genius Report software
Kit content
  • Ready-to-use reagents for Real time PCR 
  • Internal control (amplification of beta-globin gene - BG)
  • Positive control (DNA containing fragments of the MBTC genome and the BG gene)
For further information
Mycobacteria are gram-positive, rod-shaped bacilli. Several species belong to this family. Some of these are harmless and others are pathogenic to humans. Of particular concern are the species responsible for human tuberculosis infection. These belong to the Mycobacterium tuberculosis complex, which includes Mycobacterium tuberculosis (also called Koch-BK-Bacillus), Mycobacterium africanum and Mycobacterium bovis. Even today, tuberculosis remains a global emergency and an immense public health problem. The most widespread and widely known form of tuberculosis is pulmonary tuberculosis, although the disease can also affect other organs (pleura, nervous system, lymph nodes, bones, and urogenital system). The infection can also occur without symptoms of illness, in which case it is referred to as latent tubercular infection.
Why use Real time PCR assay for MBTC?
Mycobacteria are characterised by a rather slow culture growth; they can be detected by microscopic examination with the use of suitable colourant systems that utilise the high lipid content of their wall. Investigation of Mycobacterium tuberculosis complex using Real time PCR is strongly recommended as a test to be carried out in parallel with examination of the culture and microscopy.

This product uses technology patented by Biosearch Technologies, licensed for use in Human Molecular Diagnostic applications.

Files to download:

Ordering Information

Code Description Package
RQ-85-4M REALQUALITY RQ-MBT Complex 50 tests
RQ-85-6M REALQUALITY RQ-MBT Complex 100 tests
RQ-85-4A REALQUALITY RQ-MBT Complex - Automatic format 50 tests
RQ-85-6A REALQUALITY RQ-MBT Complex - Automatic format 100 tests