REALQUALITY RS-HSV 2 is a diagnostic CE-IVD kit for the identification and quantification of the DNA of Herpes simplex virus type 2 (HSV 2) by Real time PCR.


  • The device is validated on DNA extracted from various samples types
  • The assay requires only 5 µL of DNA extracted
  • If used in combination with the REALQUALITY RQ-HSV 2 STANDARD, it allows the quantification of the viral DNA present in the sample 
  • The assay shares the same thermal profile of the REALQUALITY Infectious diseases kits
  • Validated on main Real time PCR instruments
  • The assay includes dUTP/UNG system for preventing carry-over contamination and a fluorescence normalizer
  • The automatic format of the assay can be used on GENEQUALITY X120 platform
  • Easy interpretation of results with AB Genius Report software
Kit content
  • Ready-to-use reagents for Real time PCR 
  • Internal control (amplification of beta-globin gene - BG)
  • Positive control (DNA containing fragments of the HSV genome and the BG gene)
For further information

Human herpes simplex virus type 1 (HSV 1) and type 2 (HSV 2) cause recurrent infections that affect skin, mouth, lips, eyes and genital mucosa, and appear as clusters of small, painful vesicles. HSV 1 usually causes gingival stomatitis, cold sores and herpetic keratitis, whereas type 2 is more often related to genital lesions.

After the first infection the herpes simplex virus remains dormant in the nerve ganglia, from which it can periodically reactivate as a result of physical or emotional stress, febrile illnesses, sunlight and immunosuppression. Infection by these viruses is normally benign, however it is sometimes related to severe clinical forms of encephalitis and viral meningitis. Neonatal infection, often associated with HSV 2, is also clinically relevant.

Why use Real time PCR assay for HSV 2?
Some clinical manifestations typical of HSV, such as cold sores, are so characteristic that they do not require confirmation by laboratory tests; on the other hand, in cases where the lesions are atypical or not directly visible (encephalitis, endocervical genital infections), the investigation of HSV viral DNA becomes an indispensable diagnostic tool, as with the monitoring of the infection in organ transplant recipients, HIV positive individuals and newborns.

This product uses technology patented by Biosearch Technologies, licensed for use in Human Molecular Diagnostic applications.

Files to download:

Ordering Information

Code Description Package
RQ-07-4M REALQUALITY RS-HSV 2 50 tests
RQ-07-6M REALQUALITY RS-HSV 2 100 tests
RQ-07-4A REALQUALITY RS-HSV 2 - Automatic format 50 tests
RQ-07-6A REALQUALITY RS-HSV 2 - Automatic format 100 tests