AMPLIQUALITY HCV TYPE PLUS v2.0

Description

Il kit AMPLIQUALITY HCV TYPE PLUS è un IVD basato sulla tecnica Reverse Line Blot, che identifica i genotipi 1-7 del virus dell’Epatite C (HCV) ed i sottotipi a e b del genotipo 1.
Il prodotto prevede una multiplex one-step RT-PCR, a partire da RNA estratto e la successiva visualizzazione su strip degli ampliconi mediante Reverse Line Blot (RLB).

Product Characteristics

• The assay requires only 10 µL of RNA extracted
• The assay includes dUTP/Cod UNG system for preventing carry-over contamination
• The automatic format of the assay can be used on different automatic instruments for Reverse Line Blot protocols
• Easy interpretation of results with HCV TYPE PLUS Strip Reader software

Kit content

Kit includes:

• Ready-to-use reagents for End-Point PCR
• Positive control
• Internal control
• Strips and reagents for amplicons visualization by Reverse Line Blot
• Transparent film for strip interpretation
• Strip collection sheet
• Tray with 8 single-use channels for Reverse Line Blot (only in the manual format)
• CD-ROM containing the interpretative software HCV TYPE PLUS Strip Reader, the User manual, the Interpretation Table

Further Information

The Hepatitis C virus (HCV) belongs to the family of Flaviviridae and is characterized by a high grade of genomic variability.
Based on phylogenetic analysis seven different genotypes have been identified.
The genomes of those genotypes differ by approx. 30%. Each genotype, itself is subdivided into various subtypes, each of which differs by approx. 20% (in regard to the genome) from the other subtypes. The subtypes are designated by lower-case letters. These subtypes comprise different variants or quasispecies characterized by mutations that occur at a certain frequency in the patient.
HCV possesses a single-strand RNA genome of a length of approx. 9400 nucleotides. The coding regions are the CORE domain, the domain that encodes the envelope proteins and the domain of nonstructural proteins. The coding regions are flanked by highly conserved untranslated regions (UTR) at the 3’ and 5’ ends. The 5’ untranslated region can provide valuable information about the HCV genotype (i.e. genotypes 1 to 5, 6a and 6b, 7). However, using only the 5’ UTR the HCV subtypes 1a and 1b cannot be unambiguously identified and the HCV subtypes 6 (not 6a and 6b) cannot be distinguished from HCV genotype 1.
Due to this fact analysis of both, the 5’UTR and the CORE region, is required for an unambiguous identification of HCV genotype and subtype.

Ordering Information