Description
The REALQUALITY Carba-Screen kit is indicated for the screening and identification of the main Ambler classes A, B and D carbapenemases responsible for resistance to carbapenem antibiotics and colistin resistance genes by Real-Time PCR.
Product Characteristics
- The assay requires only 5 µL of DNA extracted
- The kit allows the screening and identification of the main Ambler classes A, B and D carbapenemases responsible for resistance to carbapenem antibiotics and colistin resistance genes by using three different mixes:
- Carba-Screen Real time mix identifies positive samples for A or D and B carbapenemases classes and for the Acinetobacter OXA genes
- Carba A+D Real time mix identifies the resistance genes belonging to A (KPC) and D (OXA-48 like), and the co-presence of genes associated with colistin resistance (MCR 1, 2, 4)
- Carba B Real time mix identifies the resistance genes belonging to B class (IMP, VIM, NDM)
- Validated on main Real-Time PCR instruments
- The assay includes dUTP/UNG system for preventing carry-over contamination
- The automatic format of the assay can be used on GENEQUALITY® automatic platforms
Kit content
Kit content:
- Ready-to-use reagents for Real-Time PCR
- Positive control (one or more, depending on the device variant):
- PC Carba-Screen
- PC Carba A+D
- PC Carba B
Further Information
Antimicrobial resistance is a growing health problem worldwide. The World Health Organization has included among its most critical priorities the development of new antibacterial agents for the treatment of Enterobacteriaceae resistant to carbapenems (CRE), as this type of resistance has minimized the usefulness of many commercially available drugs, limiting the therapeutic options available.
The main mechanism of resistance to carbapenems is based on the production of the carbapenemase enzyme, that is able to hydrolyze carbapenems and other β-lactam antibiotics. The encoding genes for this enzyme are associated with plasmids and therefore horizontally transferable and they are included in three of the four classes of β-lactamases (A, B and D), according to the Ambler classification.
KPC class A enzymes are currently the predominant carbapenemases reported worldwide among Enterobacteriaceae. VIM, IMP and NDM are the most geographically widespread metallo-β-lactamases. As for class D carbapenemases, OXA-48-like carbapenemases are the most common among Enterobacteriaceae.
Together with CRE, Acinetobacter baumannii is considered as one of the main hospital-acquired pathogens capable of resisting the main antimicrobials and surviving in the healthcare environment. OXA-type carbapenemases constitute the most widespread mechanism of resistance to carbapenems in this species.
Another resistance that has emerged in recent years is colistin resistance, which is considered a problem due to the lack of alternative antibiotics. Some bacteria, including Pseudomonas aeruginosa, Acinetobacter baumannii, and members of Enterobacteriaceae, such as E. coli, Salmonella spp. and Klebsiella spp. have acquired resistance to colistin. Colistin is an effective antibiotic for the treatment of most multidrug-resistant Gram-negative bacteria. Horizontal transfer of colistin resistance is given by mcr genes.
Ordering Information
| Code | Product | PKG |
|---|---|---|
| RQ-170-6M C1 | REALQUALITY Carba-Screen (screening mix) | 50 tests |
| RQ-170-6A C1 | REALQUALITY Carba-Screen (screening mix) - Automatic format | 100 tests |
| RQ-170-4M C3 | REALQUALITY Carba-Screen (identification mix) | 50 tests |
| RQ-170-4A C3 | REALQUALITY Carba-Screen (identification mix) - Automatic format | 100 tests |