REALQUALITY RS-FACTOR II G20210A

Description

The REALQUALITY RS-FACTOR II G20210A is an IVD for the detection and genotyping of the G20210A mutation in the human gene coding for the coagulation Factor II by Real-Time PCR.

Product Characteristics

• The assay requires only 1 µL of DNA extracted
• Validated on main Real time PCR instruments
• The assay includes dUTP/UNG system for preventing carry-over contamination and a fluorescence normalizer
• The automatic format of the assay can be used on GENEQUALITY^® automatic platforms
• It includes an automatic interpretation of results with AB SNP Report software

Kit content

Kit content:

• Ready-to-use reagents for Real-time PCR amplification
• Homozygous (I/I and D/D) and heterozygous (I/D) positive controls

Further Information

Thrombophilia is commonly defined as any acquired or hereditary disorder associated with an increased risk to develop thromboembolic phenomena. These phenomena occur when blood circulation is blocked by clots, originating in veins or derived from a thrombus in another area of the body. Most commonly, thrombi develop in superficial or deep veins of the legs, but can also be found in veins of the brain, retina, liver or in mesenteric veins.
Hereditary thrombosis is associated with an onset at earlier age, due to the presence of one or more genetic defects caused by gene-to-gene and/or gene-to-environment interactions.
Genetic alterations of different blood components may directly or indirectly influence blood homeostasis, thus triggering a prothrombotic state. Such alterations may lead to the loss of function of natural anticoagulants
(e.g. Protein C, Protein S, Antithrombin), to an increased activity of procoagulant factors (e.g. Prothrombin, Factor V, Factor VII, Factor IX, Factor XIII, MTHFR, MTRR) or to diminished fibrinolytic activity (e.g. PAI-1, TAFI).

Prothrombin, or Factor II, is the inactive precursor of Thrombin. Prothrombin G20210A appears to be an important risk factor for cerebral-vein thrombosis and to act synergistically with oral contraceptives.

Ordering Information