Description
The REALQUALITY EHEC/EPEC/EIEC is an IVD for the identification of Enterohemorrhagic Escherichia coli (EHEC), Shiga toxin producing Escherichia coli (STEC), Enteropathogenic Escherichia coli (EPEC) and Enteroinvasive Escherichia coli (EIEC)/Shigella by Real-Time PCR.
Product Characteristics
- The device is validated on DNA extracted from various samples types
- The assay requires only 5 µL of DNA extracted
- Validated on main Real-Time PCR instruments
- The automatic format of the assay can be used on GENEQUALITY® platform
- Easy interpretation of results with AB Genius Report software
Kit content
The kit includes:
- Freeze-dried reagents for Real-Time PCR amplification
- Extraction control (freeze-dried)
- Positive control (freeze-dried)
- Negative control
- Rehydration Buffer (solution to reconstitute the freeze-dried products)
- Water RNAse/DNAse free
Further Information
Escherichia coli is a rod-shaped, Gram-negative DNA bacterium classified as a member of the Enterobacteriaceae family. The Escherichia coli species includes not only commensal strains, but also pathogenic strains that cause a variety of human diseases, resulting in more than 2 million deaths each year. There are six well-studied intestinal E. coli pathotypes: Shiga toxin-producing E. coli (STEC), enteropathogenic E. coli (EPEC), enterotoxigenic E. coli (ETEC), enteroaggregating E. coli (EAEC), diffusely adherent (DAEC) and enteroinvasive E. coli (EIEC). These strains are classified according to their virulence properties and pathogenic mechanisms that cause gastrointestinal diseases such as diarrhea.
Although culture methods are still widely used in diagnostic laboratories, multiplex PCR has shown sensitivity and specificity for the most common categories of diarrheal E. coli strains of different clinical origins and different genotypes and serotypes. Simultaneous amplification of virulence genes typical of different E. coli pathotypes can be performed and this technique can be applied to the diagnosis of patients with hemolytic uremic syndrome, those involved in foodborne outbreaks, and patients with sporadic enterocolitis. Molecular assays based on Real-Time PCR technology are therefore indicated for the identification of these pathogens, thanks to their rapid response times, high sensitivity and specificity and are now a routine tool in diagnostic laboratories.
Ordering Information
| Code | Product | PKG |
|---|---|---|
| RQ-157-6A | REALQUALITY EHEC/EPEC/EIEC- Automatic format | 96 tests |