Description
The REALQUALITY ETEC/EIEC is an IVD for the identification of enterotoxigenic Escherichia coli (ETEC) and enteroinvasive Escherichia coli (EIEC)/Shigella by Real-Time PCR.
Product Characteristics
- The device is validated on DNA extracted from various samples types
- The assay requires only 5 µL of DNA extracted
- Validated on main Real-Time PCR instruments
- The automatic format of the assay can be used on GENEQUALITY®
- Easy interpretation of results with AB Genius Report software.
Kit content
The kit includes:
- Freeze-dried reagents for Real-Time PCR amplification
- Extraction control (freeze-dried)
- Positive control (freeze-dried)
- Negative control
- Rehydration Buffer (solution to reconstitute the freeze-dried products)
- Water RNAse/DNAse free
Further Information
Escherichia coli is a DNA bacterium, Gram-negative, rod-shaped, aerobic and facultative anaerobic, non-sporing, lactose-fermenting, DNA bacterium that grows at the optimum temperature of 37°C and is classified as a member of the Enterobacteriaceae family. The E. coli species includes not only commensal strains but also pathogenic strains that cause a variety of human diseases, resulting in more than 2 million deaths each year. Among the main pathotypes of E. coli that cause gastroenteritis are: Shiga toxin-producing E. coli (STEC), enteropathogenic E. coli (EPEC), enterotoxigenic E. coli (ETEC), enteroaggregating E. coli (EAEC), diffusely adherent E. coli (DAEC) and enteroinvasive E. coli (EIEC). These strains are classified according to their virulence properties and pathogenic mechanisms that cause gastrointestinal diseases such as diarrhea.
Although culture methods are still widely used in diagnostic laboratories, multiplex PCR has shown sensitivity and specificity for the most common categories of diarrheal E. coli strains of different clinical origins and different genotypes and serotypes. Simultaneous amplification of virulence genes typical of different E. coli pathotypes can be performed and this technique can be applied to the diagnosis of patients with the hemolytic uremic syndrome, those involved in foodborne outbreaks, and patients with sporadic enterocolitis. Molecular assays based on Real-Time PCR technology are therefore indicated for the identification of these pathogens, thanks to their rapid response times, their high sensitivity and specificity and are now a routine tool in diagnostic laboratories.
Ordering Information
| Code | Product | PKG |
|---|---|---|
| RQ-156-6A | REALQUALITY ETEC/EIEC - Automatic format | 96 tests |