REALQUALITY RQ-BKV v2.0

Description

The REALQUALITY RQ-BKV v2.0 is an IVD device for detection and quantification of the DNA of BK virus (BKV) by Real-Time PCR.

Product Characteristics

• The device is validated on DNA extracted from various samples types
• The assay requires only 5 µL of DNA extracted
• If used in combination with the REALQUALITY RQ-BKV STANDARD, it allows the quantification of the viral DNA present in the sample
• The assay shares the same thermal profile of the REALQUALITY Infectious diseases kits
• Validated on main Real-Time PCR instruments
• The assay includes dUTP/UNG system for preventing carry-over contamination and a fluorescence normalizer
• The automatic format of the assay can be used on GENEQUALITY® automatic platforms
• Easy interpretation of results with AB Genius Report software

Kit content

Kit content:

• Ready-to-use reagents for Real-Time PCR
• Internal control
• Positive control

Further Information

The BK virus (BKV) belongs to the Polyomaviridae family and is widely spread among the population with up to 90% of adults showing seroconversion and lifelong presence of specific antibodies. Generally, the primary infection is developed during childhood and is usually asymptomatic.
After the first infection, BKV remains latent in the cells of the urogenital tract and other sites (ureter, brain, spleen, B lymphocytes). In some case the BK virus becomes reactivated. This reactivation is often asymptomatic. A relatively high frequency of reactivation has been found in pregnant women (5-10 %) and immunosuppressed patients. Particularly in latter patients, the BKV infection is linked to different pathologic conditions such as hemorrhagic cystitis, interstitial nephritis, ureteral stenosis, disseminated vasculopathies, bladder cancer and multiple organ failure.
Interstitial nephritis linked to a BKV infection (BKVAN) represents a prominent cause for renal dysfunction after kidney transplant, occurring in 1-10 % of transplant recipients.
The most common BKV associated condition (20-30 % of cases) in recipients of allogeneic bone marrow transplant is the late on-set hemorrhagic cystitis (HC). Viruria is present before, during and after the cystitis and can also persist after the cure.

Ordering Information