REALQUALITY RQ-HPV HR Multiplex

Description

The REALQUALITY RQ-HPV HR Multiplex kit is an IVD for detection of the DNA of 14 high-risk genotypes of Human Papillomavirus (HPV) and genotyping of HPV 16 and HPV 18 by Real-Time PCR

Product Characteristics

• The device is validated on DNA extracted from various samples types
• The assay requires only 5 µL of DNA extracted
• The kit allows the detection of 14 high risk oncogenic HPV genotypes (HPV 16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66 e 68)
• The kit is able to specifically identify the HPV 16 and HPV 18 genotypes.
• The assay shares the same thermal profile of the REALQUALITY Infectious diseases kits and of the REALQUALITY STI kits
• Validated on main Real time PCR instruments
• The assay includes dUTP/UNG system for preventing carry-over contamination and a fluorescence normalizer
• It includes an automatic interpretation of results with AB Genius Report software
• It is also available in automatic version on GENEQUALITY® automatic platforms

Kit content

Kit content:

• Ready-to-use reagents for Real time PCR
• Positive control
• Internal control

Further Information

Human Papillomavirus (HPV) infection is the most common and widespread of sexually transmitted diseases. The different types of HPV are generally divided into low-risk and high-risk cancer risk genotypes. The latter (in particular HPV 16 and 18) are recognized as the necessary cause of cervical cancer and, in general, among the most important carcinogenic viruses for the human species. The transforming role, in fact, does not end in genesis of the cervical carcinoma, but it is strongly correlated to other neoplasms of the female and male genital sphere (vulva, vagina, anus, penis) and extragenital neoplasms (oral cavity, pharynx, larynx). The carcinogenic potentials can be ascribed to two viral oncoproteins (E6 and E7) which, after the integration of the viral genome into the host genome, cause an uncontrolled proliferative stimulus. This leads to a decrease capability in controlling the cellular surveillance mechanisms, with consequent accumulation of genetic abnormalities, increased genomic instability and the appearance of aneuploidy.
During the process of viral integration, part of the HPV DNA may be lost. However, this process never involves the E6 and E7 genes, so using a screening system targeting those genes exclude the possibility of false negative results due to viral integration events.

Ordering Information